IL-4 Monoclonal Antibody (MP4-25D2), Biotin, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Supplier:  Invitrogen 13704881

Catalog No. 50-121-57

Description

Description: The MP4-25D2 antibody reacts with human interleukin-4 (IL-4), a 15-19 kDa cytokine secreted by Th2 cells. Applications Reported: The MP4-25D2 antibody has been reported for use in detection of human IL-4 by ELISA and for intracellular staining for flow cytometry. Applications Tested: The MP4-25D2 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human Interleukin-4 (IL-4) in combination with the purified 8D4-8 (14-7049) antibody for capture and recombinant human IL-4 (14-8049) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 500 pg/mL - 4 pg/mL should be included in each ELISA plate. Filtration: 0.2 μm post-manufacturing filtered.

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

Specifications

• Antigen: IL-4
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P05112
• Gene Symbols: Il4
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 3565
• Content And Storage: 4° C, do not freeze
• Form: Liquid

• Applications: ELISA
• Clone: MP4-25D2
• Conjugate: Biotin
• Gene: Il4
• Gene Alias: B cell growth factor 1; B_cell stimulatory factor 1; B-cell growth factor 1; B-cell IgG differentiation factor; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; cytokine; H-IL-4; IGG1 induction factor; IL2; IL4; Il-4; Il4e12; ILN; Interleukin; interleukin 4; interleukin 4 variant 2; interleukin 4 variant IL-4delta2; interleukin 4 variant IL-4delta3; interleukin 4 variant IL-4int2A; interleukin 4 variant IL-4int3A; interleukin 4 variant IL-4int3B; Interleukin4; interleukin-4; interleukin-4 precursor; lymphocyte stimulatory factor 1; MGC79402; M-IL-4; Pitrakinra
• Host Species: Rat
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ

For Research Use Only.