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Invitrogen™ IL-5RA/CD125 Human ELISA Kit

Sandwich ELISA Kit

Manufacturer:  Invitrogen™ EHIL5RA

Encompass

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Catalog No. EHIL5RA


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Description

Description

The Human Interleukin 5 Receptor Alpha/CD125 (Hu IL-5RA) ELISA quantitates Hu IL-5RA in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-5RA. Principle of the method The Human IL-5RA solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Interleukin 5 (IL-5) is a soluble T cell-derived factor, also known as T cellreplacing factor (TRF), that induces B cell and eosinophil growth and differentiation. IL-5 exerts its biological activity through the IL-5 receptor (IL-5R),which is composed of two chains: an α chain that binds IL-5 with low affinity and a β chain that does not bind IL-5, but together with the IL-5 α chain,constitutes the high affinity IL-5 receptor. The cytoplasmic domain of both the α and β chains is essential for signal transduction. Specifically, the membrane-proximal proline-rich sequence of the ytoplasmic domain of the IL-5R receptor α subunit, IL-5Rα, is critical for the IL-5 induced proliferative response, expression of nuclear proto-oncogenes and tyrosine phosphorylation of cellular proteins, such as JAK1 and JAK2.
Specifications

Specifications

This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL- 11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-
96 Assays
Other proteins
HRP
0.164-40 ng/mL
Protein assays and analysis, protein biology, ELISA, ready-to-use immunoassay
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s) and stop solution
Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL
1 hr. 20 min.
Human
<10%
Human
150 pg/mL
Colorimetric
ELISA Kit
Colorimetric Microplate Reader
Biotin
Q01344
CD125 (IL5RA) ELISA Kit, 960 Assays, Human Species, Colorimetric Detection Method, 3568.0 Gene ID, Cell Culture Supernatants, Plasma, Serum Sample Type, IL5RA Gene Symbols, 150pg/mL Sensitivity, Used For Protein Assays and Analysis
3568
RUO
2°C to 8°C
4 hr. 45 min.
<12%
96 Tests
Plasma, Serum, Supernatant
CD125,CDw125,HSIL5R3,IL5R
SDS
Documents
Product Certifications
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For Research Use Only. Not for use in diagnostic procedures.