IL-6 Polyclonal Antibody, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Pepro Tech Inc 500P2650UG

Catalog No. 500P2650UG

Description

AA Sequence of recombinant protein: PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-6. Anti-Human IL-6-specific antibody was purified by affinity chromatography employing an immobilized Human IL-6 matrix. Sandwich ELISA: To detect Human IL-6 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IL-6 (500-P26Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-6. Western Blot: To detect Human IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IL-6 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

IL-6 Polyclonal antibody specifically detects IL-6 in Human samples. It is validated for ELISA, Western Blot

Specifications

• Antigen: IL-6
• Classification: Polyclonal
• Formulation: PBS with no preservative
• Gene Accession No.: P05231
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 3569
• Content And Storage: -20°C

• Applications: ELISA, Western Blot
• Conjugate: Unconjugated
• Gene: Il6
• Gene Alias: B-cell differentiation factor; B-cell hybridoma growth factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CHIL-6; CTL differentiation factor; cytokine; HGF; H-IL-6; HSF; hybridoma growth factor; Ifnb2; IFN-beta-2; Il6; IL-6; ILg6; ILN; interferon beta-2; Interleukin; interleukin 6; Interleukin 6 (interferon, beta 2); interleukin 6 precursor; interleukin BSF-2; interleukin HP-1; Interleukin6; Interleukin-6; interleukin-6 precursor; interleukin-6 protein; M-IL-6; prointerleukin 6; R-IL-6
• Immunogen: E.coli-derived Recombinant Human IL-6
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Human
• Form: Lyophilized