GE Healthcare illustra AutoSeq G-50
Remove fluorescent dye-terminators from sequencing reactions prior to analysis in an automated sequencer. GE Healthcare illustra AutoSeq G-50 columns are pre-filled with Sephadex G-50 for effective purification to ensure high-quality sequencing results.
Manufacturer: GE Healthcare 27534003
Descriptionillustra AutoSeq G-50 columns are specifically designed to remove residual fluorescent dye-terminators that can obscure data in automated sequencing. illustra AutoSeq G-50 consists of MicroSpin columns containing Sephadex G-50, pre-equilibrated in double-distilled water. This is important because even the small amount of salt in traditional buffers (such as TE buffer) can cause electrophoretic artifacts on salt-sensitive automated sequencers.
- For rapid removal of unincorporated fluorescent dye-terminators from cycle sequencing reactions prior to automated sequencing
- Columns are prepacked with Sephadex G-50 DNA Grade F and pre-equilibrated in double-distilled water with 0.05% Kathon CG/ICP Biocide added as a preservative
- Columns that are ready to use enable sample application to collection of purified product in less than 4 minutes
- Can also be used to clean up reactions prepared with the BigDye Terminator v3.1 Cycle Sequencing Kit from Applied Biosystems.
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|12 to 25μL|
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