Thermo Scientific Imject Maleimide-Activated BSA
Prepare hapten-carrier conjugates with cysteine-containing peptides and other thiolated antigens using this sulfhydryl-reactive bovine serum albumin.
Manufacturer: Thermo Scientific 77116
Thermo Scientific Imject Maleimide-Activated BSA is sulfhydryl-reactive bovine serum albumin for hapten-carrier conjugation with cysteine-containing peptides and other thiolated antigens.Purified, carrier-grade BSA is rendered sulfhydryl-reactive by chemical modification with Sulfo-SMCC crosslinker, and the resulting maleimide-activated protein is then stabilized and lyophilized in phosphate buffer. Maleimide groups form covalent crosslinks with sulfhydryl (-SH) moieties on cysteine residues of peptides and other thiol-containing haptens. Several antigen molecules can be conjugated to each BSA molecule to increase their immunogenicity and likelihood of eliciting immunized animals to produce high-titer antisera to the epitopes of interest. Select from among several package sizes of activated BSA protein, or choose the complete conjugation kit that includes optimized buffers and trouble-free spin desalting columns.
Convenient packages choose stabilized, lyophilized Maleimide-Activated BSA or complete immunogen conjugation kit
Optimized activation specifications require that each lot is activated with 15 to 20 moles of sulhydryl-reactive maleimide per mole of BSA, ensuring consistently high levels of conjugation
Sulfhydryl-reactive engineered for conjugating cysteine-containing peptide antigens to make effective immunogens for high-titer antibody production
Simple, rapid procedure complete the hapten-carrier conjugation reaction in two hours to yield sufficient immunogen for several injections and booster immunizations of host animals
High-quality carrier protein pure BSA (Fraction V) is activated using high-quality Thermo Scientific Sulfo-SMCC crosslinker and formulated to ensure high stability and consistent performance
Immunogenic and soluble moderately immunogenic and highly soluble, BSA is useful for preparation of the primary immunogen or as an irrelevant hapten-carrier for antibody screening and immunoassays after using KLH as the carrier protein to generate the immune response against the hapten
Kit contains stabilized, lyophilized Maleimide-Activated BSA (in PBS with stabilizer), buffers and desalting columns to easily prepare ready-to-use immunogens for immunization
|Upon receipt store product at 4°C. Product is shipped at ambient temperature.|
|Imject Maleimide Activated Bovine Serum Albumin|
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