MilliporeSigma™ Immobilon™ GO for Simple Immunodetection
Immobilon™ GO applies the principles of lateral flow assays for walk away processing of Western blots. Simply place your miniblot in the device and then add your wash and antibody solutions to the ports in the top. Return later to apply detection substrate and image your Western blot.
The Immobilon™ GO device is a hands-free immunodetection device designed for walk away and stand-alone processing of Western blots. Each device can process one mini blot. The unique design uses continuous sequential fluid convection across a flow matrix to enable hands free antibody binding and membrane wash steps performed during immunodetection of western blots.
The Immobilon™ GO device is intended for one-time use and does not require an external power or vacuum source for operation. Standard non-fat dry milk blot buffer is used throughout the Immobilon™ GO set up to allow for easy transition from traditional western blotting protocols. Western blots are processed in as little as 3 hours; however, for greater convenience blots may remain in the Immobilon™ GO device longer, even for overnight processing.
Compatible Blocking Solutions
- TBS-T with NFDM
Compatible Detection Reagents
- Immobilon Western HRP Substrates
- Commercially available detection reagents
- PVDF (Immobilon membranes)
|Western blotting, Dot blot, Immunoblotting (Western)|
|Chemiluminescent, Fluorescent, Chromogenic|
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