G-Biosciences™ Immunotag™ Porcine TXB2 (Thromboxane B2) ELISA Kit

A Sandwich ELISA Kit for the detection of Porcine TXB2.

Supplier:  G-Biosciences™ IT6333

Catalog No. 50-148-7821

Description

The Immunotag™ Porcine TXB2 (Thromboxane B2) ELISA is based on a sandwich enzyme-linked immunosorbent assay (ELISA) technology. A 96-well plate is coated with a specific antibody against the protein of interest (TXB2 ). During the reaction, TXB2 in the sample or standard competes with a fixed amount of TXB2 on the plate for sites on the Biotinylated Detection Antibody specific to TXB2 ), which will bind the protein and this is detected by a biotin conjugated antibody against the protein of interest. The biotin moiety is subsequently detected, following washing, by the addition of streptavidin coupled horseradish peroxidase (HRP). The supplied TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is directly proportional to the concentration of protein of interest captured on the plate.

Quality Assurance: Due to their limited shelf life, the ImmunoTag™ ELISA kits are not typically stocked as finished goods. Please allow 2-3 weeks for delivery. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision and is reflected in the manual supplied with the kit (online manuals are for reference only). In the event of a significant change the order would be confirmed with the customer before shipping

Specifications

• Assay Range: 1.563 to 100 pg/mL
• Detection Method: Sandwich ELISA, Double Antibody
• Host Species: Porcine
• Synonym: 11-DH-TXB2(11-Dehydro-thromboxane B2), 11-dehydro-TXB2

• Assay Sensitivity: 0.938 pg/mL
• Gene Symbol: TXB2
• Quantity: 1 x 96-well plate