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Integrin alpha 4 beta 7 (LPAM-1) Rat anti-Mouse, APC, Clone: DATK32 (DATK-32), eBioscience™ Non-distribution product as customer accommodation.

Rat Monoclonal Antibody

Manufacturer:  Invitrogen 17588782

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Catalog No. 50-112-3153


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Description

Description

Description: The DATK32 monoclonal antibody binds to a combinatorial epitope of mouse LPAM-1 (alpha 4/ beta 7), which is a heterodimer of the the 154 kDa alpha 4 subunit and the 130 kDa beta 7 subunit. In mouse, the beta 7 subunit is found on the majority of mature lymphocytes and a small population of thymocytes and bone marrow cells. LPAM-1 is an integrin involved in the transendothelial migration of lymphocytes across high endothelial venules (HEV), and is know to bind several ligands including MAdCAM-1, VCAM-1 and fibronectin. Binding of the DATK32 monoclonal antibody has been shown to induce aggregation of the CD8+ T cell lymphoma TK1, and block LPAM-1-mediated cell adhesion. Applications Reported: This DATK32 (DATK-32) antibody has been reported for use in flow cytometric analysis. Applications Tested: This DATK32 (DATK-32) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.

Integrin beta 7 associates with integrin alpha 4 (CD49d) to form the alpha 4 beta 7 integrin LPAM-1, expressed on intraepithelial lymphocytes. It also associates with alpha E (CD103) to form the alpha E beta 7 integrin HML-1, expressed on T cells adjacent to mucosal epithelium and intraepithelial lymphocytes. Ligands for LPAM-1 include VCAM-1 (CD106), MAdCAM-1 and fibronectin, while the ligand of HML-1 is E-cadherin (CD324). Integrin beta 7 plays an important role in the adhesion of leukocytes to endothelial cells promoting the transmigration of leukocytes to extravascular spaces during the inflammatory response.
Specifications

Specifications

Integrin alpha 4 beta 7 (LPAM-1)
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q00651, P26011
ITGB7, Gut homing receptor beta subunit, Integrin alpha-4/beta-7, Peyer patches-specific homing receptor LPAM-1, Itga4
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
DATK32 (DATK-32)
APC
Itga4
Liquid
Itga4, Itgb7
IgG2a, kappa
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
16401, 16421
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