AccuPrime™ Pfx DNA Polymerase Non-distribution product as customer accommodation.

Provides higher fidelity than Pfu DNA polymerase

Manufacturer: Invitrogen 12344032

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Catalog No. 12-344-032

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    Fidelity (vs. Taq) Fidelity (vs. Taq): 26X
    Gel Compatibility Gel Compatibility: Low
    Includes AccuPrime Pfx DNA Polymerase (500μL, at 2.5U/μL) is provided with 10X AccuPrime reaction mix (5 x 1mL) that contains dNTPs and a separate vial (2 x 1mL) of 50mMMgSO4. Reagents are provided for 1000 amplification reactions of 50μL each.
    Quantity 1000 reactions
    Storage Requirements Store at -20°C.

    AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcusspecies KODwith proofreading (3'-5' exonuclease) activity. Platinumanti-PfxDNA polymerase antibodies inhibit polymerase activity, providing hot-start capabilities for improved PCR specificity. Thermostable AccuPrime accessory proteins enhance specific primer-template hybridization during every cycle of PCR, increasing specificity, yield, and robustness over PlatinumPfx alone.

    • Achieve greater than 26 times higher fidelity than Taq DNA polymerase
    • Amplify fragments up to 12 kb
    • Minimize PCR optimization
    • Built-in hot start

    Amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR. AccuPrime Pfx DNA Polymerase is especially effective where very high levels of fidelity are required.

    Amplification of Bisulfite-Treated DNA, High Fidelity PCR, Hot Start PCR, Methylation Analysis, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Non-Coding RNA Research

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