AccuPrime™ Taq DNA Polymerase System Non-distribution product as customer accommodation.

Suitable for wide variety of applications, including multiplex PCR, TOPO TA Cloning™, and allele-specific amplifications

Manufacturer: Invitrogen 12339024

 View all options for this product

Catalog No. 12-339-024

  Check Availability
Add to Cart


    Exonuclease Activity 5' - 3'
    Fidelity 2 X
    GC-Rich PCR Performance Low
    Includes AccuPrime™ Taq DNA Polymerase is provided in a 1000 reaction kit. Each kit includes: AccuPrime™ Taq DNA Polymerase (500μL, at 2U/μL); 10X AccuPrime™ PCR Buffer I (2 x 1.25mL); 10X AccuPrime™ PCR Buffer II (2 x 1.25mL); 50mM Magnesium Chloride (500μL). 10XAccuPrime™ PCR Buffer I is designed for small genomic DNA amplicons (≤200 bp), cDNA, or plasmids. 10X AccuPrime™ PCR Buffer II is designed for genomic DNA (200 bp–4 kb). 10X AccuPrime™ PCR Buffer contains 200mM Tris-HCl (pH 8.4), 500mM KCl, 15mM MgCl2, 2mM dGTP, 2mM dATP, 2mM dTTP, 2mM dCTP, thermostable AccuPrime™ protein, 10% glycerol.
    No. of Reactions 1000.0
    Overhang 3'-A
    Polymerase AccuPrime™ Taq DNA Polymerase
    Size (Final Product) 5 kb or less
    Storage Requirements Store at -20°C.

    The AccuPrime Taq DNA Polymerase System provides reagents for amplification of nucleic acid templates with antibody-mediated, hot-start protocol for improved PCR specificity over other hot-start DNA polymerases. Platinumanti-Taq DNA polymerase antibodies inhibit polymerase activity, providing an automatic hot-start, while a thermostable accessory protein enhances specific primer-template hybridization during every cycle of PCR. This combination improves fidelity of Taq DNA Polymerase by two-fold and is ideal for high-throughput screening and multiplex PCR. AccuPrime Taq DNA Polymerase broadens primer annealing temperatures, providing optimal performance between 55°C and 65°C.

    • Specificity: Control mispriming at every cycle to get single-band PCR products
    • Ease of use: minimize reaction optimization and primer set redesign
    • Convenience: conveniently assemble reactions at room temperature

    • AccuPrime buffers: 10X AccuPrime buffers contain thermostable AccuPrime protein, Mg++, and deoxyribonucleotide triphosphates at concentrations
    • Two individual buffer systems (10X AccuPrime PCR Buffer I and II) are provided for amplification of specific types of templates
    • For small genomic DNA amplicons (≤200bp), cDNA, or plasmids

    Amplification of Bisulfite-Treated DNA, Hot Start PCR, Methylation Analysis, Multiplex PCR, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Non-Coding RNA Research, Routine PCR

  • Description & Specifications

Related Products

Product Content Feedback

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit