Invitrogen BioPrime Plus Array CGH Genomic Labeling Module
Enables reproducible labeling of genomic DNA samples for array-based Comparative Genomic Hybridization (CGH)
Manufacturer: Invitrogen 18095014
BioPrime Plus Array CGH Genomic Labeling Module is part of a system that supports:
High yields of fluorescently labeled genomic DNA
detection of gene copy number variations.
These systems combine a highly concentrated exo-Klenow fragment of DNA polymerase I and random primers to effectively label genomic targets for sensitive genomic profiling experiments. Exo-Klenow is a mutant of the large fragment of the DNA polymerase I holoenzyme that has both 5' - 3;' and 3' - 5' exonuclease activity removed. The lack of exonuclease activity makes this enzyme ideal for use in random priming protocols, enabling robust yields and efficient incorporation of Alexa Fluor AHA fluorescent nucleotides. In the BioPrime Plus Array CGH Genomic Labeling Systems, random octamers anneal to template DNA, providing priming sites for the exo- Klenow enzyme. Alexa Fluor AHA modified nucleotides (direct labeling) or amino-allyl modified nucleotides (indirect labeling) are incorporated as the polymerase extends from the priming sites. The labeled samples are then purified to remove contaminants prior to denaturing and hybridization to a microarray (direct labeling systems) or coupled to the Alexa Fluor NHS ester (indirect labeling systems) prior to hybridization. Available in a direct labeling format.
Array CGH, ChIP-on-Chip, Chromatin Biology, DNA and RNA Purification and Analysis, DNA Labeling, Gene Expression Analysis and Genotyping, Genotyping and Genomic Profiling, Nucleic Acid Labeling and Oligo Synthesis, RNAi, Epigenetics and Non-Coding RNA Research
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Shipping Condition: Labeling Module, Dry Ice; Purification Module, Room Temperature
|Module, Genomic Labeling|
|The BioPrime Core Module is found in all standard BioPrime and BioPrime Plus Systems and Modules. It contains exo-Klenow fragment (40 U/μL), 2.5X random primer solution (octamers), control DNA (Salmon Sperm), stop buffer, and distilled water. The BioPrime Plus Systems and Modules contain the Alexa Fluor modified dNTP mixes or amine modified dNTP mixes and the Alexa Fluor NHS Esters The standard BioPrime and BioPrime Plus Array CGH Genomic Labeling Systems also contain purification columns and purification buffers|
|Array CGH, ChIP-on-Chip, Chromatin Biology, DNA and RNA Purification and Analysis, DNA Labeling, Gene Expression Analysis and Genotyping, Genotyping and Genomic Profiling, Nucleic Acid Labeling and Oligo Synthesis, RNAi, Epigenetics and Non-Coding RNA Research|
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For Research Use Only. Not for use in diagnostic procedures.