|First-Strand cDNA Synthesis, RT-PCR|
|25μL cloned AMV RT (15U/μL), 500μL 5X cDNA synthesis buffer, 250μL 0.1M DTT, 100μL 10mM dNTP mix, 25μL RnaseOUT (40U/μL), 25μL oligo(dT)20 (50μM), 50μL random hexamers (50ng/μL), 1.25mL DEPC-treated water, product profile sheet|
|25 reactions (375 units)|
|113 to 131deg.F|
|Store at -70deg.C|
|45 to 55deg.C|
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