Invitrogen™ Colorimetric ReadiLAMP™ Kit, SARS-CoV-2

The Invitrogen Colorimetric ReadiLAMP Kit for SARS-CoV-2 is a loop-mediated isothermal amplification (LAMP)-based solution for rapid SARS-CoV-2 detection in saliva and nasopharyngeal/nasal swab samples.

Supplier:  Invitrogen™ A52544

Catalog No. A52544

Description

• The Invitrogen Colorimetric ReadiLAMP Kit for SARS-CoV-2 is a loop-mediated isothermal amplification (LAMP)-based solution for rapid SARS-CoV-2 detection in saliva and nasopharyngeal/nasal swab samples
• The first protocol option provides a workflow for RNA purification followed by a colorimetric LAMP assay
  • For robust SARS-CoV-2 RNA isolation, the MagMAX Viral/Pathogen II Nucleic Acid Isolation Kit (Cat. No. A48383) or PureLink Viral RNA/DNA Mini Kit (Cat. No. 12280050) are recommended
• The second protocol option consists of a simple SARS-CoV-2 viral lysis method for compatible sample types followed by a colorimetric LAMP assay
  • This protocol does not require nucleic acid isolation or complex sample preparation; instead it directly incorporates the saliva or nasopharyngeal/nasal swab samples as LAMP assay input

Features of the Colorimetric ReadiLAMP Kit for SARS-CoV-2 include:

• Simplicity - Detection is fast, simple, and intuitive; LAMP reaction changes color from purple to blue if SARS target is present
  • No instrumentation required, except for a 65 heating block
• Sensitivity - Input of up to 50% volume of purified RNA per LAMP reaction, enabling maximal sensitivity in SARS-CoV-2 detection
  • Direct LAMP: ≤250 copies per reaction (25 µL)
  • Purified RNA-based LAMP: ≤100 copies per reaction (25 µL)
• Speed - Both protocols can be completed within 1 hour
  • Direct LAMP: ∼30 minutes using crude saliva or swabs in non-serum-containing transport media
  • Purified RNA-based LAMP: ∼ 60 minutes (∼30 minutes RNA purification, followed by 30 minutes LAMP assay)
• Throughput - Process between 1 and 96 samples at a time, enabling the processing of more samples with same personnel headcount, using individual tubes, tube strips, or 96-wells

Specifications

• Bead Type: Kit does not contain beads. Recommending the use of RNA extraction MVP II kit which contains magnetic beads
• Color: During reaction color changes from purple to blue
• Sample Type: Saliva or Nasopharyngeal/Nasal Swab
• For Use With (Application): SARS-CoV-2 surveilance
• For Use With (Equipment): Heating block
• Key Functions: Detects SARS-CoV-2 presence
• Tested For: SARS-CoV-2 presence

• Detection Limit: Direct LAMP: <250 copies/25 μL LAMP reaction; Purified RNA-based LAMP: <100 copies/25 μL LAMP reaction
• Detection Method: Colorimetric readout
• Enzyme: SuperScript IV & proprietary Bst-derived DNA polymerase
• Formulation: Liquid. 5 components included in the kit.
• Includes: LAMP Buffer (5x), Enzyme Mix (10x), SARS-CoV-2 primer mix (5x), Human Control primer mix (5x), SARS-CoV-2 control RNA
• Sample Volume: Range of sample inputs
• No. of Reactions: 1000