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Invitrogen™ One Shot™ TOP10 Electrocomp™ E. coli

Supplied at transformation efficiency of 1 x 109 cfu/ μg supercoiled DNA that's ideal for high-efficiency cloning and plasmid propagation

$302.00 - $526.00

Specifications

Format One Shot
For Use With (Application) Transformation, Cloning
Product Line One Shot™
Product Type E. coil
Competent Cell Type Electrocompetent
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
C404052
Encompass
View Documents
Invitrogen™
C404052
20 Reactions
Each for $526.00
Add to cart
 
C404050
Encompass
View Documents
Invitrogen™
C404050
10 Reactions
Each for $302.00
Add to cart
 
Description

Description

Provides stable replication of high copy number plasmids. The genotype of TOP10 cells is similar to that of DH10B™ strain.

  • hsdR for efficient transformation of unmethylated DNA from PCR amplifications
  • mcrA for efficient transformation of methylated DNA from genomic preparations
  • lacZΔM15 for blue/white color screening of recombinant clones
  • endA1 for cleaner preparations of DNA—get better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I
  • recA1 for reduced occurrence of nonspecific recombination in cloned DNA
  • Bacterial/Yeast Strain: TOP10

Cloning, Transformation

Order Info

Shipping Condition: Dry Ice

Specifications

Specifications

One Shot
One Shot™
Electrocompetent
Yes
Yes
Yes
May be used for plasmids > 20 kb
Dry Ice
E. coli
High Efficiency (> 10^9 cfu⁄µg)
Not High-throughput Compatible (Manual)
Transformation, Cloning
E. coil
Not Suitable for cloning unstable DNA
Lacks F' Episome
TOP10
Not for ccdB vector propagation
Not Suitable for preparing unmethylated DNA
Yes
No
> 1x10^10
SDS
Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

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