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Invitrogen™ pBAD-DEST49 Gateway™ Destination Vector

Designed for rapid cloning with Gateway entry clone using lambda phage site-specific recombination and subsequent high-level, tightly regulated expression in E. coli

Manufacturer:  Invitrogen™ 12283016

Encompass

Catalog No. 12-283-016


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Includes: The pBAD-DEST49 Gateway destination vector includes 6μg of vector.

Description

Description

  • araBAD promoter for tightly regulated expression in E. coli
  • HP-thioredoxin fusion for improved protein yield and solubility
  • Enterokinase cleavage site for efficient cleavage of N-terminal fusion with EKMax™
  • C-terminal V5 and 6xHis tags for efficient detection and purification of fusion proteins
  • R sites for efficient recombination with any attL-flanked Gateway entry vector

Bacterial Expression/Cloning/Gateway Cloning/Protein Expression/Proteins, Expression, Isolation and Analysis

Specifications

Specifications

Inducible
araBAD
None
Ampicillin (AmpR)
His Tag (6x), V5 Epitope Tag, Thioredoxin
pBAD, pDEST
Arabinose
Gateway System Destination Expression Vector
The pBAD-DEST49 Gateway™ destination vector includes 6 µg of vector. Store at -20°C. The vector is guaranteed stable for 6 months when properly stored.
EK (Enterokinase) Recognition Site
Gateway™
Gateway™
SDS
Documents
Product Certifications
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For Research Use Only. Not for use in diagnostic procedures.