Platinum™ Blue PCR SuperMix Non-distribution product as customer accommodation.

Premixed reagents for high-throughput applications

Manufacturer: Invitrogen 12580015

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Catalog No. 12-580-015

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    Specifications

    For Use With (Application) Hot Start PCR, PCR, PCR and Real-Time PCR, Routine PCR
    Includes 4 x 1.125mL Composition: Each tube contains 22U/mL complexed recombinant Taq DNA Polymerase with Platinum Taq Antibody, 22mM Tris-HCl (pH 8.4), 55mM KCl, 1.65mM magnesium chloride, 220μM dGTP, 220μM dATP, 220μM dTTP, 220μM dCTP, stabilizers, glycerol, and blue tracking dye.
    No. of Reactions 100.0
    Overhang 3'-A
    Quantity 100 reactions
    Size (Final Product) 5kb or less

    Platinum Blue PCR SuperMix provides qualified reagents for the amplification of nucleic acid templates by PCR, and includes blue tracking dye for subsequent gel analysis. The reagents are premixed for high-throughput applications as well as general ease of use.

    Using Platinum Blue PCR SuperMix
    The mixture contains recombinant Platinum Taq DNA polymerase, Mg++, dNTPs, glycerol (used as a precipitant), and blue tracking dye. Platinum Blue PCR SuperMix is supplied at a 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solutions. Reagents are provided sufficient for 100 or 1,000 amplification reactions of approx. 50µL each.

    Hot-start specificity
    Specific binding of the anti-Taq antibody inhibits polymerase activity at room temperature. Activity is restored after a denaturation step in PCR cycling at 94°C, providing an automatic “hot start” in PCR and improving specificity and yield.

    Hot Start PCR, PCR, PCR and Real-Time PCR, Routine PCR

  • Description & Specifications

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