Invitrogen™ Platinum™ Taq DNA Polymerase

Convenient and reliable “hot start” thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase

Supplier:  Invitrogen™ 10966018

Catalog No. 10-966-018

Includes: Each box contains: Platinum Taq DNA Polymerase; 10X PCR Buffer (no magnesium chloride); 50mM magnesium chloride; KB Extender

Description

Use Platinum Taq DNA Polymerase for the amplification of DNA from complex genomic, viral, and plasmid templates, as well as in RT-PCR. The “hot start” property of the enzyme preparation is conferred by thermolabile monoclonal antibodies that render Taq DNA polymerase inactive until the initial PCR denaturation step.

• Convenience: assemble PCR reactions at room temperature
• Specificity: “hot start” kinetics reduce nonspecific primer annealing, improving product yield
• Product size: use for PCR products up to 10kb
• Flexibility: optional KB Extender gives more versatility in PCR assays

Using PlatinumTaq DNA Polymerase

• Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase has a nontemplate-dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends, and has a 5'→3' exonuclease activity (but not 3'→5' exonuclease activity)
• PCR products generated with PlatinumTaq DNA Polymerase may be used in the same downstream applications without protocol modifications

Amplification of Bisulfite-Treated DNA, Chromatin Biology, Chromatin Immunoprecipitation (ChIP), Fast PCR, Hot Start PCR, Methylation Analysis, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Noncoding RNA Research, Routine PCR

Order Info

Shipping Condition: Approved for shipment on wet or dry ice

Specifications

• Content And Storage: Each box contains:
  • 24 μl Platinum™ Taq DNA Polymerase
  • 1.25 mL 10X PCR Buffer (no magnesium chloride)
  • 1 mL 50 mM magnesium chloride
  • 1.3 mL KB Extender
  
  Store at -30°C to -10°C in a non-frost-free freezer.
• Format: Tube
• Polymerase: Platinum Taq DNA Polymerase
• Exonuclease Activity: 5' - 3'
• Product Type: DNA Polymerase
• Shipping Condition: Approved for shipment on Wet or Dry Ice
• Fidelity (vs. Taq): 1 X
• No. of Reactions: 120 Reactions
• Reaction Format: Separate Components
• Starting Material: DNA

• Detection Method: Primer-probe
• GC-Rich PCR Performance: Low
• Reaction Speed: Fast or Standard
• Product Line: Platinum™
• Quantity: 120 Reactions
• Sufficient For: 120 Reactions
• Hot Start: Built-In Hot Start
• Overhang: 3'-A
• Size (Final Product): 5 kb or less

For Research Use Only. Not for use in diagnostic procedures.