Platinum™ Taq DNA Polymerase Non-distribution product as customer accommodation.

Convenient and reliable “hot start” thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase

Manufacturer: Invitrogen 10966018

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Catalog No. 10-966-018

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    Specifications

    Includes 24μL Platinum Taq DNA Polymerase; 1.25mL 10X PCR Buffer (no magnesium chloride); 1mL 50mM magnesium chloride; 1.3mL KB Extender
    No. of Reactions 120.0
    Overhang 3'-A
    Polymerase Platinum™ Taq DNA Polymerase
    Quantity 120 reactions
    Size (Final Product) 10 kb or less
    Storage Requirements Store at -30° to -10°C. Do not store in a frost-free freezer.

    Use Platinum Taq DNA Polymerase for the amplification of DNA from complex genomic, viral, and plasmid templates, as well as in RT-PCR. The “hot start” property of the enzyme preparation is conferred by thermolabile monoclonal antibodies that render Taq DNA polymerase inactive until the initial PCR denaturation step.

    • Convenience: assemble PCR reactions at room temperature
    • Specificity: “hot start” kinetics reduce nonspecific primer annealing, improving product yield
    • Product size: use for PCR products up to 10kb
    • Flexibility: optional KB Extender gives more versatility in PCR assays

    Using PlatinumTaq DNA Polymerase

    • Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase has a nontemplate-dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends, and has a 5'3' exonuclease activity (but not 3'5' exonuclease activity)
    • PCR products generated with PlatinumTaq DNA Polymerase may be used in the same downstream applications without protocol modifications

    Amplification of Bisulfite-Treated DNA, Chromatin Biology, Chromatin Immunoprecipitation (ChIP), Fast PCR, Hot Start PCR, Methylation Analysis, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Noncoding RNA Research, Routine PCR

  • Description & Specifications

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