Invitrogen™ Platinum™ PCR SuperMix High Fidelity

Platinum™ PCR SuperMix, High Fidelity, is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification.

Supplier:  Invitrogen™ 12532016

Catalog No. 12-532-016

Includes: Platinum PCR SuperMix High Fidelity is supplied at 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solutions. Reagents sufficient for 100 amplification reactions of 50μL each are provided (4 x 1.125mL). Platinum PCR SuperMix High Fidelity Components: 22U/mL complexed recombinant Taq DNA polymerase, Pyrococcus species GB-D thermostable polymerase, and Platinum Taq Antibody; 66mM Tris-SO₄ (pH 8.9); 19.8mM (NH₄)₂SO₄; 2.4mM MgSO₄; 220μM dNTPs; and stabilizers.

Description

Platinum™ PCR SuperMix, High Fidelity, is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. You need only add template and primers, reducing set-up time by half (see figure).

With Platinum™ PCR SuperMix, High Fidelity, you will:
• Achieve greater than six-times higher fidelity than Taq DNA polymerase
• Amplify fragments up to 15 kb
• Produce products that are mixed blunt and 3-A' ends; however, the majority of ends will have a 3-A' overhangs
• Minimize PCR optimization

Using Platinum™ PCR SuperMix, High Fidelity
Platinum™ PCR SuperMix, High Fidelity, is for high-specificity, high-fidelity DNA amplification applications, such as cloning or mutagenesis. High fidelity is provided by a mixture of Taq DNA polymerase and the proofreading Pyrococcus species GB-D polymerase. High specificity is achieved by anti-Taq antibodies, allowing for automatic 'hot-start' PCR. This hot-start capability increases specificity and yield and allows for room-temperature reaction assembly.

Order Info

Approved for shipment on Wet or Dry Ice

Specifications

• Content And Storage: Platinum PCR SuperMix High Fidelity (4 x 1.125 mL)
  
  Store at -20°C in a non-frost-free freezer.
• Polymerase: Platinum Taq DNA Polymerase High Fidelity
• Shipping Condition: Wet or Dry Ice
• Concentration: 1.1X
• Hot Start: Built-In Hot Start
• Overhang: Mixed
• Size (Final Product): 15 kb or less

• GC-Rich PCR Performance: Low
• Reaction Speed: Standard
• For Use With (Application): Hot-start PCR, High-fidelity PCR
• Fidelity (vs. Taq): 6X
• No. of Reactions: 100 Reactions
• Reaction Format: SuperMix or Master Mix

For Research Use Only. Not for use in diagnostic procedures.