Platinum SuperScript CellsDirect One-Step qRT-PCR Kit with ROX
RNA purification step not needed, saves time and money
Manufacturer: Invitrogen 11754100
|10mL Resuspension Buffer; 1mL Lysis Enhancer; 500μL DNase I, Amplification Grade (1U/μL); 160μL 10X DNase I Buffer; 400μL 25mM EDTA; 100μL SuperScript III RT/Platinum Taq Mix; 2 x 1.25mL 2X Reaction Mix with ROX; 1mL 50mM MgSO4; 2mL DEPC-treated water; 10μL HeLa Total RNA (10ng/μL)|
|7000 System, 7300 System, 7700 System, 7900HT Fast System, 7900HT System, Eppendorf™ MasterCycler™ RealPlex|
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