Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase

Designed for sensitive, reproducible, end-point detection and analysis of RNA molecules by RT-PCR

Supplier:  Invitrogen™ 12574018


 View more versions of this product

Catalog No. 12-574-018

Add to cart



The SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase uses a convenient one-step formulation to perform both cDNA synthesis and PCR amplification in a single tube using gene-specific primers, and target RNAs from either total RNA or mRNA. The system uses a mixture of SuperScript III Reverse Transcriptase and Platinum Taq DNA polymerase in an optimized reaction buffer, and it can detect a wide range of RNA targets, from 200 bp to 4.5kb. The amount of starting material can range from 0.01pg to 1μg of total RNA.

  • Sensitive—routine detection down to 0.01pg total RNA
  • Convenience—one-step format for speed, convenience, and less reaction-to-reaction variability
  • Specificity—uses SuperScript III RT for cDNA synthesis up to 55°C, for more specific priming with gene specific primers
  • Amplicon size—compatible detection of targets up to 4.5kb inlength for greater flexibility

SuperScript III Reverse Transcriptase SuperScript III Reverse Transcriptase is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability. The enzyme can synthesize cDNA at a temperature range of 45° to 60°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. Because SuperScript III RT is not significantly inhibited by ribosomal and transfer RNA, it can be used to synthesize cDNA from total RNA. Platinum Taq DNA polymerase Platinum Taq DNA polymerase is recombinant Taq DNA polymerase complexed with a proprietary antibody that blocks polymerase activity at ambient temperatures. Activity is restored after the denaturation step in PCR cycling at 94°C, providing an automatic “hot start” in PCR for increased sensitivity, specificity, and yield. Final product size: 4.5 kb or less.

One-Step RT-PCR, PCR & Real-Time PCR, RT-PCR, Reverse Transcription

Order Info

Shipping Condition: Dry ice



• SuperScript III RT/Platinum Taq Mix, 50 μL
• 2X Reaction Mix, 1 mL
• Magnesium Sulfate, 500 μL (5 mM)

Store components at –30°C to –10°C.

PCR Amplified cDNA
25 Reactions
Platinum Taq
30 min.
Up to 4.5 kb
1-Step RT-PCR
Gel Electrophoresis
1-Step RT-PCR Kit
Built-In Hot Start
1-Step RT-PCR
Premixed Components
Reverse Transcription
50 μL
SuperScript III
Dry Ice


Product Certifications


Provide Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit

For Research Use Only. Not for use in diagnostic procedures.