Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins.
- Over a wide pH range–optimal activity between 6.5 and 9.5
- Under denaturing conditions–e.g., in the presence of SDS or urea
- In the presence of metal chelating agents–e.g., EDTA
- At comparatively high temperatures–optimum digestion temperature is 65°C
- Protein Filter: P
Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization.
Performance and quality testing
Endodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.
One mAnson unit is described as that amount of enzyme that liberates 1µmole of Folin-positive amino acid within one minute at 37°C using hemoglobin as a substrate.
Approved for shipment at Room Temperature or on Wet Ice
|Approved for shipment at Room Temperature or on Wet Ice|
For Research Use Only. Not for use in diagnostic procedures.
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