|cDNA Synthesis, RT-PCR (Endpoint), Real-Time PCR|
|Resuspension Buffer (1mL ); RNaseOUT Recombinant Ribonuclease Inhibitor (40 units/μL) 200μL; DNase I (1U/μL) 500μL; 10X DNase I Buffer 160μL; 25mM EDTA 120μL; Oligo(dT)(20) (50μM) 120μL; 10mM dNTP Mix 100μL; SuperScript III RT (200 units/μL) 100μL; 5X RT Buffer 600μL; 0.1M DTT 100μL; E. coli RNase H (2U/μL) 100μL; HeLa Total RNA (10ng/μL) 10μL; Forward Control Primer (10μM) 10μL; Reverse Control Primer (10μM) 10μL; E. coli RNase H (2U/μL) 100μL; HeLa Total RNA (10ng/μL) 10μL|
|Store at -20deg.C|
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