Terminal Deoxynucleotidyl Transferase, Recombinant Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Performance and quality tested

Manufacturer: Invitrogen 10533065

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Catalog No. 10-533-065

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    Includes 5X Buffer [500mM potassium cacodylate (pH 7.2), 10mM CoCl2 , 1mM DTT]
    Quantity 500 units
    Storage Requirements Store at -20°C

    Terminal Deoxynucleotidyl Transferase, Recombinant (rTdT) is DNA polymerase that catalyzes addition of deoxynucleotides to 3fi hydroxyl terminus of DNA.

    • Source: Purified from baculovirus clone of calf thymus TdT
    • Performance and Quality Testing: Endonuclease, 3' and 5' exodeoxyribonuclease, and levels of incorporation tested
    • Unit Definition: One unit incorporates 1nmol dATP into acid-precipitable material in 1 hr. at 37°C, using d(pA)50 as primer
    • Unit Reaction Conditions: 0.2M potassium cacodylate (pH 7.2), 10mM MgO4 C4 H6 , 1mM 2-mercaptoethanol, 0.5mg/mL BSA, 100 flM d(pA)50, 1mM [3H]dATP, and enzyme in 0.15mL for 1 h at 37°C

    Cloning; Restriction Enzyme Cloning; Homopolymer tailing of vector and insert for cloning; Labeling oligonucleotides with biotin, 32P- or 35S-label; Apoptosis (TUNEL)

  • Description & Specifications

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