|First-Strand cDNA Synthesis, RT-PCR|
|Random Primers, Oligo dT Primers|
|Store at -20deg.C|
|50° to 65°C|
|100μL ThermoScript RT (15U/μL); 500μL 5X cDNA Synthesis Buffer; 250μL 0.1M DTT; 2 × 250μL 10mM dNTP Mix; 100μL RNaseOUT (40U/μL); 100μL Oligo (dT)20 (50μM); 250μL Random Hexamers (50ng/μL); 1.25mL DEPC-Treated Water; 2 × 50μL E. coli Rnase H (2U/μL)|
|122° to 149°F|
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