Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
Our pricing system is unavailable. You are viewing list price.
Please call 1-800-766-7000 to place your order or try our site again later.

Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, without competent cells Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Provides a highly efficient, 5-minute, one-step cloning strategy (“TOPO Cloning”) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for subcloning

Manufacturer:  Invitrogen™ 450641

Catalog No. 45-064-1



Includes: Topoisomerase I-activated pCR™2.1-TOPO™ vector; PCR buffer; Salt solution; dNTPs; Control template; M13 forward and reverse primers; Control PCR primers; Sterile water

Description

Description

Each kit uses the pCR™ 2.1-TOPOTA vector with convenient restriction sites for subcloning. The cloning kits are available with a variety of competent cells (available separately) or without competent cells, depending upon your needs and budget. Resistant to Kanamycin (KanR) and Zeocin™ (ZeoR).
pCR™ 2.1-TOPO; TA vector: cloning and subcloning simplicity
The pCR 2.1-TOPO TA vector is linearized with 3'-thymidine (T) overhangs for direct ligation of Taq polymerase–amplified PCR products (TA cloning) and is “activated” with covalently bound Topoisomerase I. There is no need to add ligase and cloning is complete in 5 minutes. EcoRI sites flanking the PCR product insertion site allow for easy excision of inserts or use any combination of 15 convenient restriction sites flanking your PCR insert for easy, directional subcloning.
pCR 2.1-TOPO TA clone selection and manipulation
The pCR 2.1-TOPO TA vector contains both ampicillin and kanamycin resistance markers and the LacZα gene for blue/white screening.
Simplified TOPO -based cloning
Using TOPO cloning technology, there is no need for PCR primers containing specific sequences, post-PCR procedures, vector preparation, or other time-intensive DNA manipulation steps. Just add your PCR reaction straight to the provided topoisomerase-charged vector, incubate 5 minutes, and transform E. coli competent cells.
Efficient cloning
With up to 95% of clones carrying the desired insert, you can screen less clones, saving time and money. The pCR 2.1-TOPO TA vector used in this kit comes with 3'-T overhangs for efficient ligation of Taq polymerase–amplified PCR products, which contain 3'-A overhangs.
The standard in cloning
When it comes to cloning, TOPO cloning technology has been a reliable partner for thousands of scientists for over ten years. Fast, simple-to-use, and efficient, TOPO cloning has been applied to many different vectors for a wide array of applications.
TOPO TA Cloning Kits for Subcloning: kit options
The TOPO TA Cloning Kit for Sequencing can be purchased with a variety of competent cells that deliver different advantages depending upon your needs:

  • General cloning: TOP10 cells (Cat. No. K4500-01, K4500-40)
  • High-efficiency cloning: TOP10 Electrocomp™ Cells (Cat. No. K4560-01, K4560-40)
  • General cloning, bacteriophage T1 resistance: DH5α-T1R cells (Cat. No. K4520-01, K4520-40)
  • Fast growth: Mach1™ -T1R Chemically Competent E. Coli (Cat. No. K4510-20)
  • Provide your own: for flexibility and to save money (Cat. No. 450641)
Two versions of the kit that include a PureLink™ Quick Plasmid Miniprep Kit (Cat. No. K4500-02 and K4510-02) for use in isolation of clean, sequencing-ready, recombinant plasmid are also available.

  • Fast and easy: go from PCR to clones in just three steps and in as little as 5 minutes hands-on time
  • Efficient: obtain up to 95% clones with correct insert
  • Proven: reliable performance for over a decade with over 4,000 citations
  • Simple: no ligase, post-PCR procedures, or PCR primers containing specific sequences are required
  • Vector: pCR 2.1-TOPO TA vector: subcloning vector with 15 convenient restriction sites flanking your insert for easy, directional subcloning
  • Cloning method: TOPO TA Cloning: Topoisomerase I–based, 5-minute ligation of PCR products with 3 ft.-A overhangs (Taq polymerase amplified) to TOPO vector with T overhangs
  • Competent cells: various options: choose from kits with either general, high-efficiency, bacteriophage T1–resistant, fast-growing competent cells (available separately) or use your own

Cloning, PCR Cloning

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

Order Info

Shipping Condition: Dry Ice

Specifications

Specifications

Kit, Cloning
Box 1: Topoisomerase I-activated pCR2.1-TOPO vector, PCR buffer, Salt solution, dNTPs, Control template, M13 forward and reverse primers, Control PCR primers, Sterile water
Cloning, PCR Cloning
25 reactions
Documents
Provide Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit