IRF7 Monoclonal Antibody (MNGPKL), PE, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Supplier:  Invitrogen 12582982

Catalog No. 50-112-2335

Description

Description: The monoclonal antibody MNGPKL reacts with mouse interferon regulatory factor 7 (IRF7), a 54 kDa transcription factor essential for induction of the antiviral innate immune response and IFN alpha production. B cells, plasmacytoid dendritic cells (pDC), and monocytes constitutively express IRF7 in the cytoplasm until activation by viral infection, double-stranded RNA or Toll-like receptor (TLR) signaling. CpG ODNs and single-stranded RNA, recognized by CD289 (TLR9) and TLR7, respectively, trigger IRF7 activation and upregulation. This activation promotes type I IFN response to disrupt pathogen and viral infection. These events cause IRF7 phosphorylation and translocation to the nucleus where it forms a homodimer to affect transcription. Applications Reported: This MNGPKL antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This MNGPKL antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL.

Interferons (IFNs) are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms. During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-a/b genes. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. IRF7 play a major role in the innate immune pathway, interacting with the Toll-like receptor (TLR) adaptor proteins MyD88 and Tirp/TRAM and functioning as an intermediate TLR4 and TLR9 signaling. There are at least four differentially spliced isoforms of IRF7, although their function has not been clearly established.

Specifications

• Antigen: IRF7
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P70434
• Gene Symbols: IRF7
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 54123
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: MNGPKL
• Conjugate: PE
• Gene: IRF7
• Gene Alias: IMD39; Interferon regulatory factor 7; interferon regulatory factor-7H; Irf7; IRF-7; IRF7A; IRF7B; IRF7C; IRF7H; IRF-7H
• Host Species: Rat
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a κ

For Research Use Only.