Description: This 3C2 monoclonal antibody reacts with mouse interferon stimulated gene-15 (ISG-15), a 15kDa ubiquitin-like protein (UBL) and cytokine. ISG-15 is translated as a precursor that is subsequently activated by an E1 enzyme, conjugated by an E2 enzyme via the C-terminal LRLRGG motif and ligated by an E3 ligase. The activity of ISG-15 is dependent on its modification of target proteins at specific lysine residues. Several viruses utilize ISG-15 and ubiquitin deconjugating proteases, while influenza B protein NS1 has been shown to specifically bind ISG-15 thereby blocking its conjugation. In addition to its role as a protein modification, ISG-15 is secreted by monocytes and lymphocytes in response to IFNalpha, beta, or gamma, as well as influenza B virus. In this role as cytokine, it has been shown to induce secretion of IFNgamma and support NK cell proliferation and cytolytic activity. Applications Reported: This 3C2 antibody has been reported for use in immunoblotting (WB). Applications Tested: This 3C2 antibody has been tested by immunoblot of Raw 264.7 cell lysates at 2 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.ISG15 is a ubiquitin-like protein that becomes conjugated to many cellular proteins upon activation by interferon-alpha (IFNA; MIM 147660) and -beta (IFNB; MIM 147640) (Zhao et al., 2005 [PubMed 16009940]).
|PBS with 0.09% sodium azide; pH 7.2|
For Research Use Only.
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