DescriptionJ Chain Monoclonal specifically detects J Chain in Human samples. It is validated for Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry-Frozen.
|Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry (Frozen)|
|Protein A purified|
|NB100-64825 reacts with human J chain as tested by ELISA using purified human Ig fragments as the solid phase target antigen. The antibody does not detect J chain in intact IgM in ELISA assays due to steric hindrance. In Western blotting techniques NB100-64825 detects J chain in IgM only in reducing conditions.|
|Western Blot 1:100-1:2000, ELISA 20 ug/ml, Immunohistochemistry 1:10-1:500, Immunoprecipitation 20 ug/ml, Immunohistochemistry-Frozen 1:200|
|Human J Chain|
|Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.|
For Research Use Only
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