Invitrogen™ Kallikrein 14 Human ELISA Kit

Sandwich ELISA Kit

Catalog Number	Mfr. No.	Quantity
EHKLK14	Invitrogen™ EHKLK14	96 Tests
EHKLK14X5	Invitrogen™ EHKLK14X5	5 x 96 Tests

Description

The Human Kallikrein 14 (Hu KLK14) ELISA quantitates Hu KLK14 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu KLK14. Principle of the method The Human KLK14 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Serine-type endopeptidase with a dual trypsin-like and chymotrypsin-like substrate specificity. May activate/inactivate the proteinase-activated receptors F2R, F2RL1 and F2RL3 and other kallikreins including KLK1, KLK3, KLK5 and KLK11. May function in seminal clot liquefaction through direct cleavage of the semenogelin SEMG1 and SEMG2 and activation of KLK3. May function through desmoglein DSG1 cleavage in epidermal desquamation a process by which the most superficial corneocytes are shed from the skin surface. May be involved in several aspects of tumor progression including growth, invasion and angiogenesis.

Specifications

• Accession Number: Q9P0G3
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: KLK-L6
• Intraassay CV: <10%
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 4 hr. 45 min.

• Assay Sensitivity: 1.5 pg/mL
• Conjugate: Biotin
• Protein Family: GCPR
• Specificity: This ELISA pair antibody detects human KLK14. Other species not determined.
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 43847
• Interassay CV: <12%
• Label or Dye: HRP
• Research Discipline: Biomarker-Related Pathways
• Synonym: KLK-L6
• Test Time: 1 hr. 20 min.