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Ki-67 Mouse anti-Canine, Human, eFluor® 615, Clone: 20Raj1, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

$139.00 - $344.00

Specifications

Antigen Ki-67
Clone 20Raj1
Target Species Canine, Human
Conjugate eFluor™ 615
Isotype IgG1, kappa
View More Specs

Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
50-245-819 Non-distribution product as customer accommodation. View Documents Invitrogen
42569980
25 μg Each for $139.00
Add to cart
 
50-245-820 Non-distribution product as customer accommodation. View Documents Invitrogen
42569982
100 μg Each for $344.00
Add to cart
 
Description

Description

Description: The monoclonal antibody 20Raj1 recognizes the human Ki-67 protein. Two isoforms of Ki-67 exist, a 345 and 395 kDa form that are expressed in dividing cells. Ki-67 is expressed in all cell types and is detectable during active phases of the cell cycle (G1, S, G2, and mitosis) but is absent from resting cells (G0). During interphase, Ki-67 expression is localized to the nucleus but redistributes to the chromosomes during mitosis and has specifically been found to associate with heterochromatin-bound proteins such as chromobox protein homolog 3 (CBX3). In studies of tumor cells, Ki-67 expression has been used as a marker for determining the fraction of proliferating cells within a given population of tumor cells. This monoclonal antibody 20Raj1 recognizes canine Ki-67. Applications Reported: This 20Raj1 antibody has been reported for use in immunocytochemical and immunohistochemical staining of paraffin-embedded tissue (IHC-P). Applications Tested: This 20Raj1 antibody has been tested by immunocytochemistry on formaldehyde-fixed and permeabilized HeLa cells, and immunohistochemistry on FFPE human skin using low pH antigen retrieval. For immunocytochemistry and immunohistochemistry, this can be used at less than or equal to 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength. A standard Alexa Fluor® 594 filter is acceptable. Excitation: 595 nm; Emission: 615 nm. Filtration: 0.2 µm post-manufacturing filtered. Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus. Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki-67 index and histologically low-grade tumors is strong. Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.
Specifications

Specifications

Ki-67
Canine, Human
IgG1, kappa
Monoclonal
Liquid
Antibody
4° C, store in dark, DO NOT FREEZE!
20Raj1
eFluor™ 615
Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Primary
Monoclonal
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
RUO

For Research Use Only.

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