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Ki-67 Rat anti-Canine, Cynomolgus Monkey, Human, Mouse, Non-human primate, Rat, Clone: SolA15, eBioscience™ Non-distribution product as customer accommodation.

Rat Monoclonal Antibody

$109.00 - $274.00

Specifications

Antigen Ki-67
Clone SolA15
Target Species Canine, Cynomolgus Monkey, Human, Mouse, Non-human primate, Rat
Conjugate Unconjugated
Isotype IgG2a, kappa
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
50-245-563 Non-distribution product as customer accommodation.
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Invitrogen
14569880
25 μg
Each for $109.00
Add to cart
 
50-245-564 Non-distribution product as customer accommodation.
View Documents
Invitrogen
14569882
100 μg
Each for $274.00
Add to cart
 
Description

Description

Description: The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein. Ki-67 is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0). Ki-67 is detected within the nucleus during interphase but redistributes to the chromosomes during mitosis. Ki-67 is used as a marker for determining the growth fraction of a given population of cells. In studies of tumor cells, the "Ki-67 labeling index" refers to the number of Ki-67 positive cells within the population and this is used to predict outcome of particular cancer types. Ki-67 has been shown to interact with the DNA-bound protein chromobox protein homolog 3 (CBX3) (heterochromatin). The SolA15 antibody also recognizes human, non-human primate and canine Ki-67. Applications Reported: This SolA15 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded (FFPE) tissue sections and frozen (paraformaldehyde fixed) sections and immunocytochemistry (ICC). Applications Tested: This SolA15 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded mouse skin with high or low pH antigen retrieval and immunocytochemistry on fixed and permeabilized C2C12 cells at less than or equal to 5 µg/mL. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered. Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus. Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki-67 index and histologically low-grade tumors is strong. Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.
Specifications

Specifications

Ki-67
Canine, Cynomolgus Monkey, Human, Mouse, Non-human primate, Rat
IgG2a, kappa
Monoclonal
Liquid
Antibody
4° C
SolA15
Unconjugated
Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
Primary
Monoclonal
PBS with 0.09% sodium azide; pH 7.2
RUO
SDS

For Research Use Only.

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