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Ki-67 Monoclonal Antibody (SolA15), eFluor™ 450, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Supplier: Invitrogen 48569880
Description
Description: The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein. Ki-67 is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0). Ki-67 is detected within the nucleus during interphase but redistributes to the chromosomes during mitosis. Ki-67 is used as a marker for determining the growth fraction of a given population of cells. In studies of tumor cells, the Ki-67 labeling index refers to the number of Ki-67 positive cells within the population and this is used to predict outcome of particular cancer types. Ki-67 has been shown to interact with the DNA-bound protein chromobox protein homolog 3 (CBX3) (heterochromatin). The SolA15 antibody also recognizes human, non-human primate and canine Ki-67. Applications Reported: This SolA15 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This SolA15 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523). This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Ki-67 Monoclonal antibody specifically detects Ki-67 in Canine, Cynomolgus Monkey, Human, Mouse, Non-human Primate, Rat samples. It is validated for Flow Cytometry.Specifications
Ki-67 | |
Monoclonal | |
0.2 mg/mL | |
PBS with 0.09% sodium azide; pH 7.2 | |
E9PVX6, P46013 | |
Mki67 | |
Affinity chromatography | |
RUO | |
100686578, 102135895, 17345, 291234, 4288 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
SolA15 | |
eFluor 450 | |
Mki67 | |
antigen identified by monoclonal antibody Ki 67; antigen identified by monoclonal antibody Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog; Antigen KI-67; Antigen KI-67 homolog; antigen KI-67; proliferation marker protein Ki-67; antigen KI-67-like; cb31; D630048A14Rik; I79_022666; Ki67; Ki-67; KIA; LOW QUALITY PROTEIN: proliferation marker protein Ki-67; marker of proliferation Ki-67; MIB-; MIB-1; Mki67; PPP1R105; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2; sb:cb31; si:ch211-250b22.7; unnamed protein product; wu:fa11g09; wu:fb57a07; wu:fi14e05 | |
Rat | |
25 μg | |
Primary | |
Canine, Cynomolgus Monkey, Human, Mouse, Non-human Primate, Rat | |
Antibody | |
IgG2a κ |
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For Research Use Only.