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Promega Klenow Fragment, DNA Polymerase I

A DNA-dependent DNA polymerase that lacks the 5′→3′ exonuclease activity of intact E. coli DNA Polymerase I but retains its 5′→3′ polymerase, 3′→5′ exonuclease and strand displacement activities.

Manufacturer:  Promega M2201

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Catalog No. PR-M2201

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  • DNA-Dependent DNA Polymerase that Lacks the 5′→3′ Exonuclease Activity
  • Use in a variety of molecular applications
  • Heat-inactivate by heating at 75°C for 10 minutes
  • Active in many Promega 1X restriction enzyme buffers
  • Supplied at a concentration of 5u/µL


Filling-in or labeling 3' ends of 5' overhanging double-stranded DNA, second-strand cDNA synthesis, DNA sequencing by the Dideoxy method
-30°C to -10°C
150 U
5 U/μL
10X Reaction Buffer: 500mM Tris HCl (pH 7.2 at 25°C), 100mM MgSO4, 1mM DTT
10mM Tris HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 50% Glycerol
Recombinant E. coli strain
DNA Polymerase I Large (Klenow) Fragment
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