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Thermo Scientific™ Klenow Fragment, exo- Promotion

Klenow Fragment, exo- is the large fragment of DNA polymerase I. Exhibits 5'3' polymerase activity, but lacks the 3'5' and 5'3' exonuclease activities.

Manufacturer:  Thermo Scientific™ EP0421

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Catalog No. FEREP0421PM

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Description

Description

Klenow Fragment, exo-, is the large fragment of DNA polymerase I . It exhibits 5'3' polymerase activity, but lacks the 3'5' and 5'3' exonuclease activities of DNA Polymerase I. The 3'5' exonuclease activity of the enzyme is eliminated by mutations in the 3'5'-exonuclease active sit.

Highlights

  • Lacks 3'5' exonuclease activity
  • Incorporates modified nucleotides (e.g., Cy3-, Cy5-, fluorescein-, rhodamine-, aminoallyl-, biotin-labeled nucleotides)
  • Active in restriction enzyme, PCR, and RT buffers
Specifications

Specifications

5U/μL
E.coli cells with a cloned DNA fragment of the mutated polA gene
300U
metal chelators, PPi, Pi (at high concentrations) (7)
68kDa monomer
Documents
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