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Thermo Scientific Klenow Fragment Promotion
Klenow Fragment is the Large Fragment of DNA Polymerase exhibiting 5'3' polymerase, 3'
5' exonuclease (proofreading) activities, but lacking 5'
3' exonuclease activity.
Manufacturer: Thermo Scientific EP0052
Description
Klenow Fragment is the large fragment of DNA polymerase I. It exhibits 5'3' polymerase activity and 3'
5' exonuclease (proofreading) activity, but lacks 5'
3' exonuclease activity of DNA polymerase I.
Highlights
- Incorporates modified nucleotides (e.g., Cy3-, Cy5-, aminoallyl-, biotin-, digoxigenin- and fluorescently-labeled nucleotides)
- Active in restriction enzyme, PCR, RT, and T4 DNA Ligase buffers
Specifications
10U/μL | |
E.coli cells with a cloned fragment of the polA gene | |
1500U |
metal chelators, PPi, Pi (at high concentrations) (8) | |
68kDa monomer |
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