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Thermo Scientific™ Klenow Fragment Promotion

Klenow Fragment is the Large Fragment of DNA Polymerase exhibiting 5'3' polymerase, 3'5' exonuclease (proofreading) activities, but lacking 5'3' exonuclease activity.

Manufacturer:  Thermo Scientific™ EP0052

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Catalog No. FEREP0052PM

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Klenow Fragment is the large fragment of DNA polymerase I. It exhibits 5'3' polymerase activity and 3'5' exonuclease (proofreading) activity, but lacks 5'3' exonuclease activity of DNA polymerase I.


  • Incorporates modified nucleotides (e.g., Cy3-, Cy5-, aminoallyl-, biotin-, digoxigenin- and fluorescently-labeled nucleotides)
  • Active in restriction enzyme, PCR, RT, and T4 DNA Ligase buffers


E.coli cells with a cloned fragment of the polA gene
metal chelators, PPi, Pi (at high concentrations) (8)
68kDa monomer
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