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Thermo Scientific™ L-Lysine-2HCl, 13C6 for SILAC

Study protein expression by mass spectrometry with mass variants of lysine, arginine and other amino acids to perform stable isotope labeling with amino acids in cell culture.

Manufacturer:  Thermo Scientific™ 89988

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Catalog No. PI89988


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Description

Description

Thermo Scientific™ Heavy and Light Amino Acids are used to specifically analyze protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC) quantification kits.

SILAC is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of “heavy“ 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins.

Highlights:

  • Efficient – 100% label incorporation into proteins of living cells

  • Flexible – different isotopes of heavy and/or light amino acids for arginine, lysine, leucine and proline enable the quantitation of peptides derived from MS-grade proteases

  • Multiplex capabilities – several alternative isotopes of arginine and lysine are available that allow the analysis of multiple treatment conditions in each experiment

  • High-quality supplements – heavy amino acids with >99% isotope purity

Specifications

Specifications

50mg
Mass Isotopes of Amino Acids
Cells
Protein
L-Lysine-2HCl, 13C6
Protein Labeling
Mass Spectrometry
Mass Spectrometer
Room Temperature
Heavy and Light Amino Acids
Safety and Handling

Safety and Handling

SDS
Documents
Product Certifications
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Promotions

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