DescriptionThis inexpensive, simple, and very accurate Lab-Aid illustrates the principles of Thin Layer Chromatography (TLC). The lab techniques involve silica-gel coated plastic slides, indicators, developers and chromatography chambers. Each student runs chromatograms of five different sample dye solutions in order to establish Rf (Rate of flow) standards. They also run chromatograms of two 'unknowns,' which are identified for the teacher in the instruction booklet. This approach gives students an opportunity to appreciate the usefulness of TLC. TLC, which is similar and complementary to paper chromatography, can also be employed to determine the purity of a system. This kit is complete for 24 students and can be performed in a single lab period.
Class size: 24 students.
|Determine The Purity of a System with Thin Layer Chromatography|
|12 developing chambers with caps, 72 tlc layers, 4 Bottles of tlc solvent, 2 vials of capillary applicators, drop-controlled bottles of one each: sudan iv, methyl violet, indigo carmine, methyl orange and methyl blue, 2 empty vials, 24 student worksheets|
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