CD44 Std./H-CAM Ab-4 Mouse Monoclonal Antibody, Epredia™

Ensure accurate, reproducible results in immunohistochemistry, western blotting, immunoprecipitation and flow cytometry experiments.

Supplier:  Epredia MS668P1

Catalog No. MS668P1

Description

CD44 is a cell surface glycoprotein expressed on lymphocytes, monocytes, and granulocytes. CD44 is also known as homing cell adhesion molecule (H-CAM), Phagocytic glycoprotein-1 (PgP-1), ECM-III, HUTCH-1, or Hermes-1. CD44 has been implicated in cell migration, in lymphocyte homing, and in tumor metastasis. Ab-4 reacts with red cells pretreated with 2-aminoethylisothiouronium bromide (AET). Digestion of intact erythrocytes by trypsin treatment leaves a 50-54 kDa protein fragment attached to the membrane, while chymotrypsin leaves a somewhat similarly sized 51-56 kDa component that are recognized by Ab-4. This antibody enhances IL-2 production by CD2-transfected murine hybridoma stimulated by JY cells while it has no significant effect on IL-2 production by Jurkat cells stimulated with CD3, PMA, or CD2. Ab-4 enhances proliferation of PBL stimulated with CD2.

Host Species: Mouse

Clone: 156-3C11

Isotype: IgG2a

Species Reactivity: Human, Baboon and Green Monkey. Does not react with rat. Others not tested.

Epitope: Resistant to digestion by trypsin and chymotrypsin

Immunogen: Stimulated human leukocytes

Molecular Weight: 80-95 kDa

Positive Control: HUVEC or HeLa cells, tonsil or esophageal CA

Cellular Localization: Membrane

Recommended for:

• Immunohistochemistry (Formalin/paraffin)
• Western Blotting
• Immunoprecipitation (Native verified)
• Flow Cytometry

Specifications

• Antigen: CD44 Std./H-CAM Ab-4
• Classification: Monoclonal
• Concentration: 200μg/mL
• Host Species: Mouse
• Quantity: 500 μL
• Research Discipline: Oncogenesis
• Target Species: Human, Non-human Primate
• Isotype: IgG2a

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot
• Clone: 156-3C11
• Conjugate: Unconjugated
• Immunogen: Stimulated human leukocytes
• Regulatory Status: IVD
• Primary or Secondary: Primary
• Form: Concentrated