Epredia™ Lab Vision™ Grb2/sem5/ASH Signaling Protein Ab-4 Mouse Monoclonal Antibody, 200µg/mL, BSA and azide

Ensure accurate, reproducible results in immunohistochemistry and immunofluorescence experiments.

Manufacturer:  Epredia™ MS1457R1

Catalog No. MS1457R1

Description

Grb2 protein contains one SH2 domain flanked on both sides by an SH3 domain. Ab-1 binds to the junction between the amino terminal SH3 domain and the SH2 domain of Grb2. Reactivity with Grb2 deletion mutants suggests that its epitope maps between aa 54-62. In response to EGF, Grb2 binds, through its SH3 domains, to the guanine nucleotide exchange factor, Sos. The Grb2-Sos complex then binds the EGF-receptor. These interactions play a critical role in the activation of the p21ras signalling pathway.

Host Species: Mouse

Clone: 2GB04 (C1.4)

Isotype: IgG1/κ

Species Reactivity: Human, Cow, Mouse, Rat and Xenopus. Others not known.

Epitope: aa 54-62

Immunogen: Purified recombinant human Grb2 protein

Molecular Weight: 24-25 kDa

Positive Control: HeLa cells, Tonsil

Cellular Localization: Cytoplasmic

Recommended for:

• Immunohistochemistry (Formalin/paraffin)
• Immunofluorescence

Note:

USA: RUO; Int'l: RUO

General References:

1. Schlessinger J. Current Opinion in Genetics and Development, 1994 Feb, 4(1):25-30.

2. Boulikas T. Critical Reviews in Eukaryotic Gene Expression, 1995, 5(1):1-77.

3. Chardin P et al. Febs Letters, 1995 Aug 1, 369(1):47-51.

4. Downward J. Febs Letters, 1994 Jan 31, 338(2):113-7.

b>General References:

1. Schlessinger J. Current Opinion in Genetics and Development, 1994 Feb, 4(1):25-30.

2. Boulikas T. Critical Reviews in Eukaryotic Gene Expression, 1995, 5(1):1-77.

3. Chardin P et al. Febs Letters, 1995 Aug 1, 369(1):47-51.

4. Downward J. Febs Letters, 1994 Jan 31, 338(2):113-7.

Specifications

• Antigen: Grb2/sem5/ASH Signaling Protein Ab-4
• Classification: Monoclonal
• Conjugate: Unconjugated
• Host Species: Mouse
• Quantity: 1 mL
• Research Discipline: Signal Transduction
• Target Species: Bovine, Human, Mouse, Rat, Xenopus
• Isotype: IgG2a κ

• Applications: Immunofluorescence, Immunohistochemistry (Paraffin)
• Clone: 2GB04; same as C1.4
• Formulation: Ready-to-use for IHC (UVLP)
• Immunogen: Purified recombinant human Grb2 protein
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Form: Prediluted