Epredia™ Lab Vision™ UltraVision™ Detection System: anti-Polyvalent

A versatile and robust biotin-streptavidin detection system for research applications and cost-conscious laboratories.

Supplier:  Epredia™ TP015AF

Catalog No. TP015AF

Description

This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody that reacts with the primary antibody, enzyme-labeled streptavidin, and substrate/chromogen.

• Labeled streptavidin-biotin immunoenzymatic antigen detection system

• Species: Goat

• Specificity: anti-Mouse IgG (H+L), anti-Rabbit IgG (H+L)

• Enzyme: Alkaline Phosphatase

• Chromogen/Substrate: Fast-Red/Naphthol Phosphate Buffer

Includes:

TP-015-AF: 15mL Ultra V Block (TA-015-UB), 15mL Biotinylated Goat Anti-Polyvalent (TP-015-BN), 15mL Streptavidin Alkaline Phosphatase (TS-015-AP), 15mL Naphthol Phosphate Substrate (TA-015-AFS), three Fast-Red Tablets (TA-003-AFC)

Note:

UltraVision detection system detects a specific antibody bound to an antigen in tissue sections. The specific antibody is located by a biotin-conjugated secondary antibody. This step is followed by the addition of a streptavidin-enzyme conjugate that binds to the biotin present on the secondary antibody. The specific antibody, secondary antibody, and streptavidin-enzyme complex is then visualized with an appropriate substrate/chromogen.

Specifications

• Chromagen: Fast-Red
• Enzyme: Alkaline Phosphatase
• Species: Goat
• Substrate: Naphthol Phosphate Substrate
• Quantity: 15mL

• Count: 75 to 150 Slides
• Product Line: Lab Vision™, UltraVision™
• Specificity: anti-Mouse IgG (H+L), anti-Rabbit IgG (H+L)
• Includes: TP-015-AF: 15mL Ultra V Block (TA-015-UB), 15mL Biotinylated Goat Anti-Polyvalent (TP-015-BN), 15mL Streptavidin Alkaline Phosphatase (TS-015-AP), 15mL Naphthol Phosphate Substrate (TA-015-AFS), three Fast-Red Tablets (TA-003-AFC)