Thermo Scientific™ Lambda Exonuclease 

Lambda Exonuclease is a highly processive exodeoxyribonuclease that selectively digests the 5'-phosphorylated strand of double-stranded DNA in 5'-3' direction.

Manufacturer:  Thermo Scientific™ EN0562

Catalog No. FEREN0562

Description

Thermo Scientific Lambda Exonuclease is a highly processive 5'=>3' exodeoxyribonuclease. It selectively digests the 5'-phosphorylated strand of double-stranded DNA. The enzyme exhibits low activity on single-stranded DNA and non-phosphorylated DNA, and has no activity at nicks and limited activity at gaps in DNA.

Highlights

• Active in Thermo Scientific PCR buffers

Applications

• Generating single-stranded PCR products for use in:
  • DNA sequencing
  • Analysis of DNA single-strand conformation polymorphism (SSCP)
  • Rolling circle amplification
   
• Producing single-stranded DNA from double-stranded DNA fragments
• Cloning of PCR products

Note

Use of this enzyme in certain applications may be covered by patents and may require a license.

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Specifications

• Concentration: 10U/μL
• Source: E.coli cells with a cloned exo gene of phage lambda
• Molecular Weight: 26kDa monomer
• Quantity: 5,000U