Laminin gamma 1 Antibody (A5) - Azide and BSA Free, Novus Biologicals™

Rat Monoclonal Antibody

Supplier:  Novus Biologicals NBP2478420.1MG

Catalog No. NBP247842A

Description

Laminin gamma 1 Monoclonal specifically detects Laminin gamma 1 in Human, Mouse, Bovine samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunofluorescence, CyTOF-ready.

Specifications

• Antigen: Laminin gamma 1
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Dilution: Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready
• Gene Alias: LAMB2Laminin-7 subunit gamma, Laminin B2 chain, laminin subunit gamma-1, laminin, gamma 1 (formerly LAMB2), Laminin-1 subunit gamma, Laminin-10 subunit gamma, Laminin-11 subunit gamma, Laminin-2 subunit gamma, Laminin-3 subunit gamma, Laminin-4 subunit gamma, Laminin-6 subunit gamma, Laminin-8 subunit gamma, Laminin-9 subunit gamma, MGC87297, S-LAM gamma, S-laminin subunit gamma
• Host Species: Rat
• Molecular Weight of Antigen: 210 kDa
• Quantity: 0.1 mg
• Research Discipline: Apoptosis, Cancer, Cytoskeleton Markers, Extracellular Matrix, Tumor Suppressors
• Gene ID (Entrez): 3915
• Target Species: Human, Mouse
• Form: Purified

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
• Clone: A5
• Conjugate: Unconjugated
• Formulation: PBS with No Preservative
• Gene Symbols: LAMC1
• Immunogen: Murine EHS laminin preparation
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Laminins are large hetero-trimeric, non-collagenous glycoproteins composed of alpha, beta, and gamma chains. This MAb reacts with laminin B2/1 chain of ∼210kDa and does not cross-react with other basement membrane components or fibronectin. Its specificity was established by immunoprecipitation and immunofluorescence of human skeletal muscle and kidney with laminin chain-specific MAbs. Epithelial sheets in vivo are separated from the mesenchymal elements of the stroma by a thin layer of a specialized type of extracellular matrix termed the basement membrane (BM). This structure consists of individual components, some of which are ubiquitous in BMs and some are not. The ubiquitous ones comprise laminin (LN), entactin/nidogen (EN), collagen type IV (CIV), and large heparan sulfate proteoglycan (HSPG), which interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG2a κ