LanthaScreen™ Tb-PY20 Antibody Kit

LanthaScreen™ Tb-PY20 Antibody Kit

Supplier:  LIFE TECHNOLOGIES PV3552

Catalog No. PV3552

Description

A general reagent to monitor tyrosine kinase activity, the pY20 antibody binds to phosphorylated tyrosines within a peptide or physiological protein substrate.

For use in combination with Fluorescein-poly-GT (PV3610) or Fluorescein-poly-GAT (PV3611) to evaluate inhibitors in medium to high throughput screening applications.

Specifications

• Antigen: Tyrosine kinases
• Conjugate: Tb (Terbium)/FITC/Lanthanide Chelates
• Quantity: 25 μg
• Research Discipline: Kinase Antibodies and Immunoassays
• Packaging: 1 Kit(s)
• Antibody Molecular Weight: 150000 Da
• Format: 384-well Plate
• Shipping Condition: Dry Ice
• Research Category: Signal Transduction
• Target Enzyme: Tyrosine Kinases
• Validated Kinases: ABL1, ABL2, AXL, BLK, BMX, BTK, CSF1R, CSK, DDR2, EGFR, EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA7, EPHA8, EPHB1, EPHB2, EPHB3, EPHB4, ERBB2, ERBB4, FER, FGFR1, FGFR2, FGFR3, FGFR4, FGR, FLT1, FLT3, FLT4, FRK, FYN, HCK, IGF1R, INSR, KDR, KIT, LCK, LTK, LYNA, LYNB, MATK, MERTK, MET, MST1R, MUSK, NTRK1, NTRK2, NTRK3, PDGFRA, PDGFRB, PTK2, PTK2B, PTK6, RET, ROS1, SRC, SRCN1, SRMS, SYK, TEC, TEK, TNK2, TTK, TYRO3, YES1, ZAP70

• Concentration: 0.45mg/mL
• Molecular Weight of Antigen: 150kDa
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Terbium-labeled anti-pY20 antibody in HEPES buffered saline. Store at -20°C.
• Assay Entry: Biochemical terbium phospho-specific activity
• Excitation/Emission: 340⁄490, 520
• Readout: End Point
• Target Entry: ABL1 G250E, CSF1R, CSK, EPHA5, KIT, PDGFRA T674I, PDGFRA V561D, PTK6, RET Y791F, SYK, TYRO3, ZAP70, FGFR1, FGFR2, BLK, EGFR, EPHB2, FGFR4, IGF1R, LYNA, MATK, MERTK, NTRK3, RET V804L, ROS1, TEK, TTK, FLT1, MUSK, SRCN1, TEC, ABL1 E255K, BMX, EPHA4, FYN, PTK2, PTK2B, RET, ABL1 T315I, EPHA3, EPHB4, FLT3, FLT4, INSR, LCK, YES1, EGFR L858R, ERBB4, KDR, MET, TNK2, ABL1, ABL2, AXL, FGFR3, FGFR3 K650E, FGR, HCK, KIT V654A, EPHA1, EPHA8, EPHB3, INSRR, LYNB, MST1R, NTRK1, PDGFRA D842V, SRMS, EGFR L861Q, EPHA2, FER, LTK, PDGFRA, PDGFRB, EGFR T790M, EPHA7, EPHB1, MET M1250T, FRK, ITK, FLT3 D835Y, SRC, ABL1 Y253F, BTK, DDR2, FES, KIT T670I, TXK, ERBB2, NTRK2
• Technique: TR-FRET
• Product Line: LanthaScreen™

For Research Use Only. Not for use in diagnostic procedures.