LIME Monoclonal Antibody (LIME-06), Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Thermo Scientific MA119365

Catalog No. 01-676-121

Description

LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.

Specifications

• Antigen: LIME
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 15mM sodium azide; pH 7.4
• Gene Accession No.: Q9H400
• Gene Symbols: Lime1
• Immunogen: Bacterially expressed intracellular fragment corresponding to aa 141-295 of human LIME
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1

• Applications: Flow Cytometry, Immunoprecipitation
• Clone: LIME-06
• Conjugate: Unconjugated
• Gene: Lime1
• Gene Alias: 2810038K19Rik; dJ583P15.4; FLJ20406; Lck interacting transmembrane adaptor 1; lck-interacting membrane protein; Lck-interacting molecule; lck-interacting transmembrane adapter 1; Lime; lime 1; LIME1; LP8067; RP4-583P15.5
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 54923
• Content And Storage: 4° C, do not freeze
• Form: Liquid