LIN28B Rabbit anti-Human, Polyclonal, Invitrogen™

Rabbit Polyclonal Antibody

Manufacturer:  Invitrogen PA549685

Catalog No. PA549685

Description

The antibody detects endogenous levels of total LIN28B protein.

Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5 ft.-GGAG-3 ft. motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5 ft.-GGAG-3 ft. motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.

Specifications

• Antigen: LIN28B
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: LIN28B
• Format: Liquid
• Gene Symbols: LIN28B
• Immunogen: Peptide sequence around aa.242∽246( P-S-V-Q-K) derived from Human LIN28B
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Polyclonal
• Target Species: Human

• Applications: Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.4
• Gene Accession No.: Q6ZN17
• Gene Alias: CSDD2
• Host Species: Rabbit
• Isotype: IgG
• Quantity: 100 μL
• Storage Requirements: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Primary or Secondary: Primary
• Gene ID (Entrez): 389421