BioVision Inc Lipoprotein Lipase Activity Fluorometric Assay Kit: High-Throughput Suitable Fluorometric Assay for measuring Lipoprotein Lipase (LPL) Activity in various biological samples such as Serum, Tissues, Cells etc. Simple, Sensitive & Accurate. 100 Assays.
Manufacturer: BioVision Inc K721100
Lipoprotein Lipase Activity Fluorometric Assay Kit: Lipoprotein lipase (LPL) is a member of the lipase family that hydrolyzes triglycerides in chylomicrons and very low-density lipoprotein (VLDL). Mutations in lipoprotein lipase can lead to a variety of disorders such as lipoprotein metabolism, hypertriglyceridemia etc. BioVision's LPL Activity Assay Kit contains a quenched substrate that fluoresces upon hydrolysis by LPL. The fluorometric intensity is directly proportional to the amount of substrate hydrolyzed. This assay detects total lipase activity when no inhibitor is used. Comparing results in the presence or absence of an LPL inhibitor allows for quantification of LPL activity specifically. Our results indicate that the majority (~90%) of lipase activity detected by this kit in post-heparin treated mouse plasma is from LPL. To determine the exact LPL specific activity in mouse plasma, measure activity in pre- and post-heparin treated plasma.
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