Invitrogen™ Rat LIX-Cell Lysate (CXCL5) ELISA Kit

ELISA

Catalog Number	Mfr. No.	Quantity
ERCXCL5CL	Invitrogen™ ERCXCL5CL	96 Tests
50-254-5373	Invitrogen™ ERCXCL5CLX5	5 x 96 Tests
50-254-5374	Invitrogen™ ERCXCL5CLX10	10 x 96 Tests

Description

The Rat LIX (CXCL5) ELISA quantitates Rt LIX in rat cell or tissue lysates. The assay will exclusively recognize both natural and recombinant Rt LIX. Principle of the method The Rat LIX solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The protein encoded by this gene is an inflammatory chemokine that belongs to the CXC chemokine family. This chemokine is produced concomitantly with interleukin-8 (IL8) in response to stimulation with either interleukin-1 (IL1) or tumor necrosis factor-alpha (TNFA). This chemokine is a potent chemotaxin involved in neutrophil activation.

Specifications

• Accession Number: P97885
• Assay Sensitivity: 15 pg/ml
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA
• Sample Type: Cell Lysates
• Sufficient For: 96 assays
• For Use With (Equipment): Colorimetric Microplate Reader
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Cell Lysis Buffer, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 20 μL
• Synonym: Cxcl5
• Test Time: 1 hrs 20 mins

• Assay Range: 8.23 to 6000 pg/mL
• Calibration Range: 15 pg/mL to 6000 pg/mL
• Conjugate: HRP
• Protein Family: Chemokine & Receptors
• Specificity: The antibody pair provided in this kit recognizes rat LIX / CXCL5.
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene Symbol: Cxcl5, Cxcl6
• Intraassay CV: <10
• Label or Dye: HRP
• Research Discipline: Immunology
• Storage Requirements: 2°C to 8°C
• Target: CXCL5
• Total Assay Time: 4 hrs 45 mins