Thermo Scientific™ Luminaris Color Probe qPCR Master Mix, high ROX

Ready-to-use qPCR master mixes with color and premixed UDG optimized for probe detection using standard cycling protocols.

Supplier:  Thermo Scientific™ K0332

Catalog No. FERK0332

Description

Thermo Scientific™ Luminaris Color Probe qPCR Master Mixes are ready-to-use qPCR master mixes with color and premixed UDG optimized for probe detection using standard cycling protocols.

Thermo Scientific Luminaris Color Probe qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template, primers and probe need to be added.

The Hot Start Taq DNA polymerase, in combination with an optimized buffer, ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control. The Luminaris Color Probe qPCR Master Mixes are supplemented with an inert blue dye and a separate Yellow Sample Buffer that contains a yellow dye. The qPCR reaction mix containing both components is green.

The use of Luminaris Color Probe qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color Probe qPCR Master Mixes with ROX at different concentrations as passive reference dye are available for most qPCR platforms.

• Specificity no primer dimers

• Sensitivitity detects low copy number targets

• Wide linear range accurate quantification across 9 orders of magnitude

• Reproducibility and convenience ready-to-use 2X master mix

• Blue Master Mix and Yellow Sample Buffer for easy pipetting
• UDG in the master mix to prevent carry-over contamination
• Excellent qPCR performance
• Versions with premixed ROX for different real-time platforms
• Standard cycling protocols

Luminaris Color Probe High ROX qPCR Master Mix (2X) and Luminaris Color Probe Low ROX qPCR Master Mix (2X) contain different amounts of ROX passive reference dye for specific real-time platforms.

Includes:

• Luminaris Color Probe qPCR Master Mixes include Hot Start Taq Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye
• 40X Yellow Sample Buffer with a yellow dye
• Nuclease-free water

Recommended for:

Gene expression analysis; siRNA validation; Genotyping; Pathogen detection

Specifications

• Content And Storage: Contains:
  • 4 x 1.25 mL Luminaris Color Probe High ROX qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG and dNTPs (with dUTP) in an optimized PCR buffer with a blue dye; sufficient for 500 x 20 μL reactions. The master mix contains high concentration of ROX passive reference dye for specific real-time platforms.
  • 1 x 1.25 mL 40X Yellow Sample Buffer
  • 4 x 1.25 mL Nuclease-Free Water
  
  Store at -20°C.
• Detection Method: Primer-probe
• GC-Rich PCR Performance: High
• Polymerase: Taq DNA Polymerase
• For Use With (Equipment): 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
• Product Type: qPCR Master Mix
• Sufficient For: 500 Reactions of 20 μL
• Concentration: 2X (Luminaris Color Probe High ROX qPCR Master Mix), 40X (Yellow Sample Buffer)
• Sample Type: DNA, dsDNA

• Description: Color Probe High ROX
• Format: Tube
• PCR Method: qPCR
• Reaction Speed: Standard
• Product Line: Luminaris
• Quantity: 500 x 20 μL Reactions
• For Use With (Application): Gene Expression, Genotyping, siRNA Validation
• No. of Reactions: 500 Reactions
• Volume: 4 x 12.5 mL

For Research Use Only. Not for use in diagnostic procedures.