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Thermo Scientific™ Luminaris Color Probe qPCR Master Mix

Optimize qPCR with probe detection using standard cycling protocols with these ready-to-use master mixes with premixed UDG, available with or without blue color.


Products 4
Catalog Number Mfr. No. Description Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Description Quantity Price Quantity & Availability  
FERK0353
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Thermo Scientific™
K0353
Color Probe 1250 x 20µL rxns This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.
N/A
FERK0351
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Thermo Scientific™
K0351
Color Probe 250 x 20µL rxns This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.
N/A
FERK0354
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Thermo Scientific™
K0354
Color Probe 5000 x 20µL rxns This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.
N/A
FERK0352
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Thermo Scientific™
K0352
Color Probe 500 x 20µL rxns This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.
N/A
Description

Description

Luminaris Color Probe and Luminaris Probe qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template, primers and probe need to be added.

The Hot Start Taq DNA polymerase, in combination with an optimized buffer, ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control. The Luminaris Color Probe qPCR Master Mixes are supplemented with an inert blue dye and a separate Yellow Sample Buffer that contains a yellow dye. The qPCR reaction mix containing both components is green.

The use of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes with ROX at different concentrations as passive reference dye are available for most qPCR platforms.

Highlights

  • Blue Master Mix and Yellow Sample Buffer for easy pipetting
  • UDG in the master mix to prevent carry-over contamination
  • Excellent qPCR performance
    • Specificity — Hot Start Taq DNA Polymerase and the optimized buffer reduce primer dimers
    • Sensitivitity — single copy detection
    • Wide linear range — accurate quantification across 9 orders of magnitude
    • Reproducibility and convenience — ready-to-use 2X master mix
    • Equal performance of blue and colorless master mixes
  • Versions with premixed ROX for different real-time platforms
  • Standard cycling protocols
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