Ly-6A/E Rat anti-Mouse, Alexa Fluor 647, Clone: E13-161.7, BD
Rat Monoclonal Antibody
Manufacturer: BD Biosciences 565355
The E13-161.7 mAb recognizes Ly-6A.2 and Ly-6E.1, which are allelic members of the Ly-6 multigene family. Ly-6A/E is also known as stem cell antigen 1 (Sca-1/Sca1), or T-cell-activating protein (TAP). Ly-6A/E is a phosphatidylinositol-anchored protein of ∽18kDa that is expressed on multipotent hematopoietic stem cells (HSC) in mice with both Ly-6 haplotypes. Sca-1+ HSC are found in the adult bone marrow and fetal liver, but not in the early embryo yolk sac or intraembryonic hematopoietic sites, and can be mobilized to the peripheral blood and spleen in the adult. In mice expressing the Ly-6.2 haplotype, Ly-6A/E is also expressed on distinct subpopulations of bone marrow and peripheral B lymphocytes, myeloid cells, and thymic and peripheral T lymphocytes, on the earliest intrathymic T-cell precursor population, and in several non-hematopoietic tissues. Strains with the Ly-6.1 haplotype have few Ly-6A/E+ resting peripheral lymphocytes, whereas activation of T cells from mice of both Ly-6 haplotypes leads to strong expression of the Sca-1 antigen. Studies with the D7 antibody have demonstrated that Ly-6A/E may be involved in the regulation of B and T lymphocyte responses, and it appears to be required for T-cell receptor-mediated T-cell activation. Purified E13-161.7 mAb can block binding of FITC-conjugated D7 antibody (anti-Ly-6A/E) to mouse splenocytes, but purified mAb D7 is unable to block binding of FITC-conjugated E13-161.7 antibody.Alexa Fluor™ 647 conjugates are highly photostable and remain fluorescent over a broad pH range. The excitation and emission maxima are nearly identical to those of APC. However, APC tends to be brighter while Alexa Fluor™ 647 is more optimal for intracellular applications. This fluorochrome exhibits uncommon photostability, making it an ideal choice for use in fluorescence microscopy. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor™ 647 cannot be used simultaneously.
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|SCA-1, Sca1, Stem cell antigen 1, TAP, T-cell-activating protein, Ly6|
|BALB/c mouse-derived “pre-T” cell hybridoma|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor™ 647 under optimum conditions, and unreacted Alexa Fluor™ 647 was removed.|
|Alexa Fluor 647|
|Rat WI, also known as Wistar (outbred) IgG2a, κ|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
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